A Micro Method for the Determination of Base in Blood and Serum and Other Biological Materials
نویسندگان
چکیده
The use of benzidine hydrochloride to precipitate sulfates as insoluble benzidine sulfate was first proposed by Miiller (1902) for the determination of free and bound sulfur in minerals. Subsequently Rosenheim and Drummond (1914) and Fiske (1921) used it for the determination of sulfates in urine. Later Fiske (1922) described a method for the determination of total base in urine, the bases after removal of phosphates being first converted to sulfates by ignition with sulfuric acid, precipitated as benzidine sulfate, the precipitate being then titrated with alkali. The method here presented is one for the determination of base in blood, serum, urine, or any biological material. Similarly to Fiske’s method the base is converted to sulfates by ignition with sulfuric acid and the equivalent sulfates are precipitated by benzidine hydrochloride. Unlike Fiske’s method where the precipitate is directly titrated with alkali, base sulfates present are determined by titrating an aliquot of the filtrate from the benzidine sulfate. From this value and the titer of the benzidine hydrochloride reagent the concentration of base is readily calculated. The reactions involved are
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